NLRP3 and mTOR Reciprocally Regulate Macrophage Phagolysosome Formation and Acidification Against Vibrio vulnificus Infection.
Identifieur interne : 000078 ( Main/Exploration ); précédent : 000077; suivant : 000079NLRP3 and mTOR Reciprocally Regulate Macrophage Phagolysosome Formation and Acidification Against Vibrio vulnificus Infection.
Auteurs : Xian-Hui Huang [République populaire de Chine] ; Yao Ma [République populaire de Chine] ; Meng-Meng Zheng [République populaire de Chine] ; Na Chen [République populaire de Chine] ; Mei-Na Hu [République populaire de Chine] ; Liu-Ying Wu [République populaire de Chine] ; Yi Zheng [République populaire de Chine] ; Yong-Liang Lou [République populaire de Chine] ; Dan-Li Xie [République populaire de Chine]Source :
- Frontiers in cell and developmental biology [ 2296-634X ] ; 2020.
Abstract
The marine bacterium Vibrio vulnificus causes potentially fatal bloodstream infections, typically in patients with chronic liver diseases. The inflammatory response and anti-bacterial function of phagocytes are crucial for limiting bacterial infection in the human hosts. How V. vulnificus affects macrophages after phagocytosis is unclear. In this report, we found that the bactericidal activity of macrophages to internalize V. vulnificus was dependent on mammalian target of rapamycin (mTOR) and NOD-like receptor (NLR) family pyrin domain containing 3 (NLRP3) interaction. Additionally, the NLRP3 expression was dependent on mTORC1 activation. Inhibited mTORC1 or absence of NLRP3 in macrophages impaired V. vulnificus-induced phagosome acidification and phagolysosome formation, leading to a reduction of intracellular bacterial clearance. mTORC1 signaling overactivation could increase NLRP3 expression and restore insufficient phagosome acidification. Together, these findings indicate that the intracellular bactericidal activity of macrophages responding to V. vulnificus infection is tightly controlled by the crosstalk of NLRP3 and mTOR and provide critical insight into the host bactericidal activity basis of clearance of V. vulnificus through lyso/phagosome.
DOI: 10.3389/fcell.2020.587961
PubMed: 33117816
PubMed Central: PMC7578225
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">NLRP3 and mTOR Reciprocally Regulate Macrophage Phagolysosome Formation and Acidification Against <i>Vibrio vulnificus</i> Infection.</title><author><name sortKey="Huang, Xian Hui" sort="Huang, Xian Hui" uniqKey="Huang X" first="Xian-Hui" last="Huang">Xian-Hui Huang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Ma, Yao" sort="Ma, Yao" uniqKey="Ma Y" first="Yao" last="Ma">Yao Ma</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Meng Meng" sort="Zheng, Meng Meng" uniqKey="Zheng M" first="Meng-Meng" last="Zheng">Meng-Meng Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Na" sort="Chen, Na" uniqKey="Chen N" first="Na" last="Chen">Na Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Mei Na" sort="Hu, Mei Na" uniqKey="Hu M" first="Mei-Na" last="Hu">Mei-Na Hu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Liu Ying" sort="Wu, Liu Ying" uniqKey="Wu L" first="Liu-Ying" last="Wu">Liu-Ying Wu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Yi" sort="Zheng, Yi" uniqKey="Zheng Y" first="Yi" last="Zheng">Yi Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Lou, Yong Liang" sort="Lou, Yong Liang" uniqKey="Lou Y" first="Yong-Liang" last="Lou">Yong-Liang Lou</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Xie, Dan Li" sort="Xie, Dan Li" uniqKey="Xie D" first="Dan-Li" last="Xie">Dan-Li Xie</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33117816</idno><idno type="pmid">33117816</idno><idno type="doi">10.3389/fcell.2020.587961</idno><idno type="pmc">PMC7578225</idno><idno type="wicri:Area/Main/Corpus">000005</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000005</idno><idno type="wicri:Area/Main/Curation">000005</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000005</idno><idno type="wicri:Area/Main/Exploration">000005</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">NLRP3 and mTOR Reciprocally Regulate Macrophage Phagolysosome Formation and Acidification Against <i>Vibrio vulnificus</i> Infection.</title><author><name sortKey="Huang, Xian Hui" sort="Huang, Xian Hui" uniqKey="Huang X" first="Xian-Hui" last="Huang">Xian-Hui Huang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Ma, Yao" sort="Ma, Yao" uniqKey="Ma Y" first="Yao" last="Ma">Yao Ma</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Meng Meng" sort="Zheng, Meng Meng" uniqKey="Zheng M" first="Meng-Meng" last="Zheng">Meng-Meng Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Na" sort="Chen, Na" uniqKey="Chen N" first="Na" last="Chen">Na Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Mei Na" sort="Hu, Mei Na" uniqKey="Hu M" first="Mei-Na" last="Hu">Mei-Na Hu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Liu Ying" sort="Wu, Liu Ying" uniqKey="Wu L" first="Liu-Ying" last="Wu">Liu-Ying Wu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Yi" sort="Zheng, Yi" uniqKey="Zheng Y" first="Yi" last="Zheng">Yi Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Lou, Yong Liang" sort="Lou, Yong Liang" uniqKey="Lou Y" first="Yong-Liang" last="Lou">Yong-Liang Lou</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Xie, Dan Li" sort="Xie, Dan Li" uniqKey="Xie D" first="Dan-Li" last="Xie">Dan-Li Xie</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in cell and developmental biology</title><idno type="ISSN">2296-634X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The marine bacterium <i>Vibrio vulnificus</i> causes potentially fatal bloodstream infections, typically in patients with chronic liver diseases. The inflammatory response and anti-bacterial function of phagocytes are crucial for limiting bacterial infection in the human hosts. How <i>V. vulnificus</i> affects macrophages after phagocytosis is unclear. In this report, we found that the bactericidal activity of macrophages to internalize <i>V. vulnificus</i> was dependent on mammalian target of rapamycin (mTOR) and NOD-like receptor (NLR) family pyrin domain containing 3 (NLRP3) interaction. Additionally, the NLRP3 expression was dependent on mTORC1 activation. Inhibited mTORC1 or absence of NLRP3 in macrophages impaired <i>V. vulnificus</i>-induced phagosome acidification and phagolysosome formation, leading to a reduction of intracellular bacterial clearance. mTORC1 signaling overactivation could increase NLRP3 expression and restore insufficient phagosome acidification. Together, these findings indicate that the intracellular bactericidal activity of macrophages responding to <i>V. vulnificus</i> infection is tightly controlled by the crosstalk of NLRP3 and mTOR and provide critical insight into the host bactericidal activity basis of clearance of <i>V. vulnificus</i> through lyso/phagosome.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">33117816</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">2296-634X</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in cell and developmental biology</Title><ISOAbbreviation>Front Cell Dev Biol</ISOAbbreviation></Journal><ArticleTitle>NLRP3 and mTOR Reciprocally Regulate Macrophage Phagolysosome Formation and Acidification Against <i>Vibrio vulnificus</i> Infection.</ArticleTitle><Pagination><MedlinePgn>587961</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fcell.2020.587961</ELocationID><Abstract><AbstractText>The marine bacterium <i>Vibrio vulnificus</i> causes potentially fatal bloodstream infections, typically in patients with chronic liver diseases. The inflammatory response and anti-bacterial function of phagocytes are crucial for limiting bacterial infection in the human hosts. How <i>V. vulnificus</i> affects macrophages after phagocytosis is unclear. In this report, we found that the bactericidal activity of macrophages to internalize <i>V. vulnificus</i> was dependent on mammalian target of rapamycin (mTOR) and NOD-like receptor (NLR) family pyrin domain containing 3 (NLRP3) interaction. Additionally, the NLRP3 expression was dependent on mTORC1 activation. Inhibited mTORC1 or absence of NLRP3 in macrophages impaired <i>V. vulnificus</i>-induced phagosome acidification and phagolysosome formation, leading to a reduction of intracellular bacterial clearance. mTORC1 signaling overactivation could increase NLRP3 expression and restore insufficient phagosome acidification. Together, these findings indicate that the intracellular bactericidal activity of macrophages responding to <i>V. vulnificus</i> infection is tightly controlled by the crosstalk of NLRP3 and mTOR and provide critical insight into the host bactericidal activity basis of clearance of <i>V. vulnificus</i> through lyso/phagosome.</AbstractText><CopyrightInformation>Copyright © 2020 Huang, Ma, Zheng, Chen, Hu, Wu, Zheng, Lou and Xie.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Xian-Hui</ForeName><Initials>XH</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Yao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Meng-Meng</ForeName><Initials>MM</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Na</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Mei-Na</ForeName><Initials>MN</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Liu-Ying</ForeName><Initials>LY</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Yi</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lou</LastName><ForeName>Yong-Liang</ForeName><Initials>YL</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Dan-Li</ForeName><Initials>DL</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Wenzhou Key Laboratory of Sanitary Microbiology, Wenzhou, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Cell Dev Biol</MedlineTA><NlmUniqueID>101630250</NlmUniqueID><ISSNLinking>2296-634X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">NLRP3</Keyword><Keyword MajorTopicYN="N">Vibrio vulnificus</Keyword><Keyword MajorTopicYN="N">acidification</Keyword><Keyword MajorTopicYN="N">bactericidal activity</Keyword><Keyword MajorTopicYN="N">mTOR</Keyword><Keyword MajorTopicYN="N">phagolysosome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>07</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>29</Day><Hour>5</Hour><Minute>54</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>30</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33117816</ArticleId><ArticleId IdType="doi">10.3389/fcell.2020.587961</ArticleId><ArticleId IdType="pmc">PMC7578225</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nat Rev Immunol. 2015 Oct;15(10):599-614</Citation><ArticleIdList><ArticleId IdType="pubmed">26403194</ArticleId></ArticleIdList></Reference><Reference><Citation>J Microbiol. 2005 Feb;43 Spec No:118-31</Citation><ArticleIdList><ArticleId IdType="pubmed">15765065</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2010 Feb;8(2):117-28</Citation><ArticleIdList><ArticleId IdType="pubmed">20075926</ArticleId></ArticleIdList></Reference><Reference><Citation>Infect Immun. 1991 Jul;59(7):2232-8</Citation><ArticleIdList><ArticleId IdType="pubmed">2050395</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Rep. 2015 Jul 7;12(1):102-115</Citation><ArticleIdList><ArticleId IdType="pubmed">26119735</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Biochem Sci. 2017 Apr;42(4):245-254</Citation><ArticleIdList><ArticleId IdType="pubmed">27932073</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2010 May 1;184(9):5287-97</Citation><ArticleIdList><ArticleId IdType="pubmed">20348425</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Host Microbe. 2018 Jun 13;23(6):766-774.e5</Citation><ArticleIdList><ArticleId IdType="pubmed">29779931</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Mol Sci. 2018 Mar 12;19(3):</Citation><ArticleIdList><ArticleId IdType="pubmed">29534520</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2012;8(3):e1002581</Citation><ArticleIdList><ArticleId IdType="pubmed">22457618</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Mol Cell Biol. 2008 Oct;9(10):781-95</Citation><ArticleIdList><ArticleId IdType="pubmed">18813294</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2015 Mar 6;290(10):6670-8</Citation><ArticleIdList><ArticleId IdType="pubmed">25586187</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2017 Jan 1;198(1):492-504</Citation><ArticleIdList><ArticleId IdType="pubmed">27881705</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2012 Apr 15;188(8):3658-66</Citation><ArticleIdList><ArticleId IdType="pubmed">22412198</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Med. 2016 May 30;213(6):877-85</Citation><ArticleIdList><ArticleId IdType="pubmed">27139490</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Cell. 2013 Dec;24(23):3736-45</Citation><ArticleIdList><ArticleId IdType="pubmed">24088573</ArticleId></ArticleIdList></Reference><Reference><Citation>Infect Immun. 2009 May;77(5):1723-33</Citation><ArticleIdList><ArticleId IdType="pubmed">19255188</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2008 Oct 15;181(8):5501-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18832707</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Metab. 2017 Feb 7;25(2):225-226</Citation><ArticleIdList><ArticleId IdType="pubmed">28178561</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2017 Jul 18;12(7):e0181454</Citation><ArticleIdList><ArticleId IdType="pubmed">28719654</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2013;4:2834</Citation><ArticleIdList><ArticleId IdType="pubmed">24280772</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 1979 Jan 4;300(1):1-5</Citation><ArticleIdList><ArticleId IdType="pubmed">758155</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Immunol. 2013 Jun;14(6):543-53</Citation><ArticleIdList><ArticleId IdType="pubmed">23644505</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2017 May 31;8:997</Citation><ArticleIdList><ArticleId IdType="pubmed">28620366</ArticleId></ArticleIdList></Reference><Reference><Citation>Infect Immun. 2014 Feb;82(2):731-44</Citation><ArticleIdList><ArticleId IdType="pubmed">24478087</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Immunol. 2019 Jan 31;10:125</Citation><ArticleIdList><ArticleId IdType="pubmed">30766538</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2015 Aug 14;10(8):e0136019</Citation><ArticleIdList><ArticleId IdType="pubmed">26274504</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Host Microbe. 2015 Jan 14;17(1):47-57</Citation><ArticleIdList><ArticleId IdType="pubmed">25590758</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2017 Jun 7;7(1):3005</Citation><ArticleIdList><ArticleId IdType="pubmed">28592812</ArticleId></ArticleIdList></Reference><Reference><Citation>F1000Prime Rep. 2014 Jul 08;6:52</Citation><ArticleIdList><ArticleId IdType="pubmed">25184042</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2009 Sep 15;183(6):3942-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19717510</ArticleId></ArticleIdList></Reference><Reference><Citation>Semin Immunol. 2015 Aug;27(4):286-96</Citation><ArticleIdList><ArticleId IdType="pubmed">26360589</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Cell Infect Microbiol. 2017 Aug 11;7:352</Citation><ArticleIdList><ArticleId IdType="pubmed">28848713</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2018 Jul 26;379(4):375</Citation><ArticleIdList><ArticleId IdType="pubmed">30044934</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Immunol. 2011;29:707-35</Citation><ArticleIdList><ArticleId IdType="pubmed">21219188</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Cell Biol. 2007 Apr;9(4):367-78</Citation><ArticleIdList><ArticleId IdType="pubmed">17351642</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Immunol. 2010 Jun;40(6):1545-51</Citation><ArticleIdList><ArticleId IdType="pubmed">20333626</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci China Life Sci. 2016 Aug;59(8):777-91</Citation><ArticleIdList><ArticleId IdType="pubmed">27430889</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2010 Jan 15;327(5963):296-300</Citation><ArticleIdList><ArticleId IdType="pubmed">20075245</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Biochim Biophys Sin (Shanghai). 2018 Sep 1;50(9):888-896</Citation><ArticleIdList><ArticleId IdType="pubmed">30060081</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2016 Sep 27;7:12874</Citation><ArticleIdList><ArticleId IdType="pubmed">27670879</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Immunol. 2018 Jun 21;9:1427</Citation><ArticleIdList><ArticleId IdType="pubmed">29977244</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):14152-7</Citation><ArticleIdList><ArticleId IdType="pubmed">22891340</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2009 Jul 15;183(2):787-91</Citation><ArticleIdList><ArticleId IdType="pubmed">19570822</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2011 Feb 02;2:12</Citation><ArticleIdList><ArticleId IdType="pubmed">21687408</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Microbiol. 2020 Jan;28(1):81-82</Citation><ArticleIdList><ArticleId IdType="pubmed">31519331</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2015 Jun 12;11(6):e1004970</Citation><ArticleIdList><ArticleId IdType="pubmed">26069969</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Host Microbe. 2017 Jun 14;21(6):719-730.e6</Citation><ArticleIdList><ArticleId IdType="pubmed">28579255</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2013 Nov 15;191(10):5230-8</Citation><ArticleIdList><ArticleId IdType="pubmed">24089192</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2008 Sep 15;181(6):3733-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18768823</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2017 Jul 24;8(1):98</Citation><ArticleIdList><ArticleId IdType="pubmed">28740109</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2013 Nov;8(11):2281-2308</Citation><ArticleIdList><ArticleId IdType="pubmed">24157548</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Microbiol. 2012 Feb;14(2):149-54</Citation><ArticleIdList><ArticleId IdType="pubmed">21995284</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Microbiol. 2018 Feb;20(2):423-430</Citation><ArticleIdList><ArticleId IdType="pubmed">29027375</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Emerg Med. 2010 May;28(4):424-31</Citation><ArticleIdList><ArticleId IdType="pubmed">20466220</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Immunol. 2009 Aug;9(8):594-600</Citation><ArticleIdList><ArticleId IdType="pubmed">19590530</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Host Microbe. 2008 Jun 12;3(6):352-63</Citation><ArticleIdList><ArticleId IdType="pubmed">18541212</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Immunol. 2015 Jan;36(1):1-2</Citation><ArticleIdList><ArticleId IdType="pubmed">25488670</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Huang, Xian Hui" sort="Huang, Xian Hui" uniqKey="Huang X" first="Xian-Hui" last="Huang">Xian-Hui Huang</name></noRegion><name sortKey="Chen, Na" sort="Chen, Na" uniqKey="Chen N" first="Na" last="Chen">Na Chen</name><name sortKey="Chen, Na" sort="Chen, Na" uniqKey="Chen N" first="Na" last="Chen">Na Chen</name><name sortKey="Hu, Mei Na" sort="Hu, Mei Na" uniqKey="Hu M" first="Mei-Na" last="Hu">Mei-Na Hu</name><name sortKey="Hu, Mei Na" sort="Hu, Mei Na" uniqKey="Hu M" first="Mei-Na" last="Hu">Mei-Na Hu</name><name sortKey="Huang, Xian Hui" sort="Huang, Xian Hui" uniqKey="Huang X" first="Xian-Hui" last="Huang">Xian-Hui Huang</name><name sortKey="Huang, Xian Hui" sort="Huang, Xian Hui" uniqKey="Huang X" first="Xian-Hui" last="Huang">Xian-Hui Huang</name><name sortKey="Lou, Yong Liang" sort="Lou, Yong Liang" uniqKey="Lou Y" first="Yong-Liang" last="Lou">Yong-Liang Lou</name><name sortKey="Lou, Yong Liang" sort="Lou, Yong Liang" uniqKey="Lou Y" first="Yong-Liang" last="Lou">Yong-Liang Lou</name><name sortKey="Lou, Yong Liang" sort="Lou, Yong Liang" uniqKey="Lou Y" first="Yong-Liang" last="Lou">Yong-Liang Lou</name><name sortKey="Ma, Yao" sort="Ma, Yao" uniqKey="Ma Y" first="Yao" last="Ma">Yao Ma</name><name sortKey="Ma, Yao" sort="Ma, Yao" uniqKey="Ma Y" first="Yao" last="Ma">Yao Ma</name><name sortKey="Wu, Liu Ying" sort="Wu, Liu Ying" uniqKey="Wu L" first="Liu-Ying" last="Wu">Liu-Ying Wu</name><name sortKey="Wu, Liu Ying" sort="Wu, Liu Ying" uniqKey="Wu L" first="Liu-Ying" last="Wu">Liu-Ying Wu</name><name sortKey="Xie, Dan Li" sort="Xie, Dan Li" uniqKey="Xie D" first="Dan-Li" last="Xie">Dan-Li Xie</name><name sortKey="Xie, Dan Li" sort="Xie, Dan Li" uniqKey="Xie D" first="Dan-Li" last="Xie">Dan-Li Xie</name><name sortKey="Xie, Dan Li" sort="Xie, Dan Li" uniqKey="Xie D" first="Dan-Li" last="Xie">Dan-Li Xie</name><name sortKey="Zheng, Meng Meng" sort="Zheng, Meng Meng" uniqKey="Zheng M" first="Meng-Meng" last="Zheng">Meng-Meng Zheng</name><name sortKey="Zheng, Meng Meng" sort="Zheng, Meng Meng" uniqKey="Zheng M" first="Meng-Meng" last="Zheng">Meng-Meng Zheng</name><name sortKey="Zheng, Yi" sort="Zheng, Yi" uniqKey="Zheng Y" first="Yi" last="Zheng">Yi Zheng</name><name sortKey="Zheng, Yi" sort="Zheng, Yi" uniqKey="Zheng Y" first="Yi" last="Zheng">Yi Zheng</name><name sortKey="Zheng, Yi" sort="Zheng, Yi" uniqKey="Zheng Y" first="Yi" last="Zheng">Yi Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RapamycinFungusV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000078 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000078 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RapamycinFungusV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:33117816
|texte= NLRP3 and mTOR Reciprocally Regulate Macrophage Phagolysosome Formation and Acidification Against Vibrio vulnificus Infection.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:33117816" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a RapamycinFungusV1
| This area was generated with Dilib version V0.6.38. |  |